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We hypothesized that phosphorylation of CAIR 1/BAG 3 would regulate its binding to PLC. EGF treatment of MDA 435 cells induced a dose dependent reduction of the binding of PLC to CAIR 1/BAG 3 (Figure 5a). The EGF dose response for this dissociation is log linear (r2=0.99) with an EC50 of 55 ng/ml.

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